Journal: The EMBO Journal
Article Title: A versatile toolbox for determining IRES activity in cells and embryonic tissues
doi: 10.1038/s44318-025-00404-5
Figure Lengend Snippet: Reagents and tools table
Article Snippet: Mouse embryonic mesenchymal C3H/10T1/2 (ATCC: CCL-226) cells or human HEK393T (ATCC: CRL-3216) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, 11965–118) containing 2 mM l -glutamine, supplemented with 10% fetal calf serum (Gibco, ES009-B), 100 U/ml penicillin and 0.1 mg/ml streptomycin (EMD Millipore, TMS-AB2-C or Gibco, 15140–122) at 37 °C in 5% CO 2 -buffered incubators.
Techniques: Recombinant, Generated, Sequencing, Cloning, Reverse Transcription, SYBR Green Assay, Protease Inhibitor, Modification, Lysis, RNAscope, Reporter Assay, Gel Extraction, Purification, Plasmid Preparation, Software, Cell Culture, Cytometry